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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
13/01/2010 |
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Data da última atualização: |
19/10/2010 |
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Autoria: |
CERQUEIRA-SILVA, C. B. M.; CARDOSO-SILVA, C. B.; NONATO, J. V. A.; CORRÊA, R. X.; OLIVEIRA, A. C. de. |
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Afiliação: |
CARLOS BERNARD MORENO CERQUEIRA-SILVA, UESB; CLAUDIO BENICIO CARDOSO-SILVA, UESB; JULIANA VIEIRA A. NONATO, UESB; RONAN XAVIER CORRÊA, UESC; ANTONIO CARLOS DE OLIVEIRA, UESB. |
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Título: |
Genetic dissimilarity of ‘yellow’ and ‘sleep’ passion fruit accessions based on the fruits physical-chemical characteristics. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 9, n. 3, p. 210-218, Sept. 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
The intra- and inter-specific genetic dissimilarity between 14 ‘yellow’ passion fruit plants and eight ‘sleep’ passion fruit plants were evaluated through nine physical-chemical descriptors, whose measured values were submitted to descriptive (average, standard deviation and variation coefficient) and inferential [univariated (ANOVA, averages and correlations tests) and multivariated (Mahalanobis distance, hierarchical clustering, Singh coefficient and Mantel test)] statistics. Intra- and, especially, inter-specific variability were found among the passion fruit plants (p < 0.001). The total soluble solid, equatorial diameter, total titratable acidity, and fruit weight descriptors presented the highest percentage of relative contribution, totaling 85.2% of the observed divergence. Preferential crossings among genotypes with physical-chemical characteristics of desirable fruits and useful genetic dissimilarity in divergent and convergent crossings were identified. |
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Palavras-Chave: |
Análise multivariada; Características físico-químicas de frutos; Diversidade genética; Diversity; Maracujeiro amarelo; Maracujeiro do-sono; Multivariated analysis; Passiflora edulis Sims; Passiflora setacea DC; Variabilidade genética. |
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Thesaurus Nal: |
Variability. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01997naa a2200301 a 4500
001 1580243
005 2010-10-19
008 2009 bl uuuu u00u1 u #d
100 1 $aCERQUEIRA-SILVA, C. B. M.
245 $aGenetic dissimilarity of ‘yellow’ and ‘sleep’ passion fruit accessions based on the fruits physical-chemical characteristics.
260 $c2009
520 $aThe intra- and inter-specific genetic dissimilarity between 14 ‘yellow’ passion fruit plants and eight ‘sleep’ passion fruit plants were evaluated through nine physical-chemical descriptors, whose measured values were submitted to descriptive (average, standard deviation and variation coefficient) and inferential [univariated (ANOVA, averages and correlations tests) and multivariated (Mahalanobis distance, hierarchical clustering, Singh coefficient and Mantel test)] statistics. Intra- and, especially, inter-specific variability were found among the passion fruit plants (p < 0.001). The total soluble solid, equatorial diameter, total titratable acidity, and fruit weight descriptors presented the highest percentage of relative contribution, totaling 85.2% of the observed divergence. Preferential crossings among genotypes with physical-chemical characteristics of desirable fruits and useful genetic dissimilarity in divergent and convergent crossings were identified.
650 $aVariability
653 $aAnálise multivariada
653 $aCaracterísticas físico-químicas de frutos
653 $aDiversidade genética
653 $aDiversity
653 $aMaracujeiro amarelo
653 $aMaracujeiro do-sono
653 $aMultivariated analysis
653 $aPassiflora edulis Sims
653 $aPassiflora setacea DC
653 $aVariabilidade genética
700 1 $aCARDOSO-SILVA, C. B.
700 1 $aNONATO, J. V. A.
700 1 $aCORRÊA, R. X.
700 1 $aOLIVEIRA, A. C. de
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 9, n. 3, p. 210-218, Sept. 2009.
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Embrapa Agricultura Digital (CNPTIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
21/11/2012 |
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Data da última atualização: |
08/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
PIERRE MARRACCINI, Cenargen, CIRAD; FELIPE VINECKY, Cenargen; GABRIEL S. C. ALVES, Cenargen; HUMBERTO J. O. RAMOS, IAPAR; SONIA ELBELT, Cenargen, CIRAD; NATALIA G. VIEIRA, Cenargen; FERNANDA A. CARNEIRO, Cenargen; PATRICIA S. SUJII, Cenargen; JEAN C. ALEKCEVETCH, Cenargen; VÂNIA A. SILVA, UFV; FÁBIO M. DAMATTA, UFV; MARIA AMELIA GAVA FERRÃO, SAPC; THIERRY LEROY, CIRAD; DAVID POT, CIRAD; LUIZ G. E. VIEIRA, SAPC; FELIPE RODRIGUES DA SILVA, CNPTIA; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford, v. 63, n. 11, p. 4191-4212, 2012. |
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DOI: |
10.1093/jxb/ers103 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Differential expression; Drought acclimation; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea Canephora; Genética. |
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Thesaurus NAL: |
Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03043naa a2200445 a 4500
001 1940133
005 2020-01-08
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1093/jxb/ers103$2DOI
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aGenetics
650 $aCafé
650 $aCoffea Canephora
650 $aGenética
653 $aCandidate gene
653 $aDifferential expression
653 $aDrought acclimation
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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